Chromatographic methods are generally used to separate a component into its individual parts, to obtain a target substance or for qualitative and quantitative analysis. Chromatography is a separation process in which 2 different immiscible phases are brought into equilibrium through different distributions.
Common types of column chromatography are axial flow chromatography and radial flow chromatography. They are used in many areas, e.g. in the food and beverage industry, in the pharmaceutical industry and in environmental analysis. Column chromatography can be used to separate proteins, enzymes, amino acids, carbohydrates, fats and many other substances.
In this presentation, the advantages and disadvantages of axial and radial flow chromatography, their areas of application and differences will be explained using a case study.