The use of hydrogen peroxide for bio-decontamination is widespread and opens up countless possibilities for application in a wide variety of areas due to its ease of use, but above all due to the fast, reliable killing of microorganisms. Since both the mode of action of H2O2 and factors influencing the killing of microorganisms during bio-decontamination are well researched and understood, technically sophisticated possibilities arise again and again for optimizing the decontamination process, among other things with regard to even shorter process times. The lecture spans the arc from the basics of H2O2 decontamination to current application examples in practice and an outlook on future challenges and opportunities.