Clean room operators are facing challenges like high operational costs, unexpected downtime and frequent delays in introducing new drug products or revised formulations. Building and verifying the digital twin of the infrastructure and performing 3D simulations for particles, airflow and energy helps create and restructure production and laboratory environments faster and at lower costs. This supports contamination control strategies as required by the European Union Good Manufacturing Practice (EU GMP) Annex 1, reduces equipment use for experiments and tests, supports predicting contamination sources and accelerates time-to-market for new or reformulated drug products. An exemplary case study including simulation analyses and validation of a lab will be demonstrated for the project PEARL.