This presentation covers key aspects of aseptic sampling. The selection of the correct valve is explained, taking into account factors such as chemical resistance, pressure and temperature requirements and the avoidance of contamination. Sampling in bottle systems is then discussed, including the correct handling and sealing of bottles to ensure sterile sampling. Practical examples of errors are given to illustrate the importance of correct technique and hygiene. Relevant certificates and standards (e.g. ISO, EHEDG) that guarantee the quality and safety of sampling are also covered.